Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus.
Identifieur interne : 003122 ( Main/Exploration ); précédent : 003121; suivant : 003123Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus.
Auteurs : Seisuke Hatakeyama [Japon] ; Yusuke Matsuoka ; Hidehiro Ueshiba ; Nobukazu Komatsu ; Kyogo Itoh ; Shigeki Shichijo ; Takao Kanai ; Masaya Fukushi ; Isao Ishida ; Teruo Kirikae ; Takehiko Sasazuki ; Tohru Miyoshi-AkiyamaSource :
- Virology [ 1096-0341 ] ; 2008.
Descripteurs français
- KwdFr :
- ARN viral (), ARN viral (génétique), Lignée cellulaire, Nucléocapside (génétique), Nucléocapside (métabolisme), Protéines de la matrice virale (génétique), Protéines de la matrice virale (métabolisme), Protéines nucléocapside (), Protéines nucléocapside (génétique), Protéines nucléocapside (métabolisme), Virus du SRAS ().
- MESH :
English descriptors
- KwdEn :
- Cell Line, Nucleocapsid (genetics), Nucleocapsid (metabolism), Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), RNA, Viral (chemistry), RNA, Viral (genetics), SARS Virus (chemistry), Viral Matrix Proteins (genetics), Viral Matrix Proteins (metabolism).
- MESH :
- chemical , chemistry : Nucleocapsid Proteins, RNA, Viral.
- chemistry : SARS Virus.
- genetics : Nucleocapsid, Nucleocapsid Proteins, RNA, Viral, Viral Matrix Proteins.
- metabolism : Nucleocapsid, Nucleocapsid Proteins, Viral Matrix Proteins.
- Cell Line.
Abstract
When expressed in mammalian cells, the nucleocapsid (N) and membrane (M) proteins of the severe acute respiratory syndrome coronavirus (SARS-CoV) are sufficient to form pseudoparticles. To identify region(s) of the N molecule required for pseudoparticle formation, we performed biochemical analysis of the interaction of N mutants and M in HEK293 cells. Using a peptide library derived from N, we found that amino acids 101-115 constituted a novel binding site for M. We examined the ability of N mutants to interact with M and form pseudoparticles, and our observations indicated that M bound to NDelta(101-115), N1-150, N151-300, and N301-422, but not to N1-150Delta(101-115). However, pseudoparticles were formed when NDelta(101-115) or N301-422, but not N1-150 or N151-300, were expressed with M in HEK293 cells. These results indicated that the minimum portion of N required for the interaction with M and pseudoparticle formation consists of amino acids 301-422.
DOI: 10.1016/j.virol.2008.07.012
PubMed: 18703211
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001A85
- to stream PubMed, to step Curation: 001A85
- to stream PubMed, to step Checkpoint: 001B45
- to stream Ncbi, to step Merge: 001D17
- to stream Ncbi, to step Curation: 001D17
- to stream Ncbi, to step Checkpoint: 001D17
- to stream Main, to step Merge: 003212
- to stream Main, to step Curation: 003122
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus.</title><author><name sortKey="Hatakeyama, Seisuke" sort="Hatakeyama, Seisuke" uniqKey="Hatakeyama S" first="Seisuke" last="Hatakeyama">Seisuke Hatakeyama</name><affiliation wicri:level="3"><nlm:affiliation>Department of Infectious Diseases, Research Institute, International Medical Center of Japan, Tokyo, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Infectious Diseases, Research Institute, International Medical Center of Japan, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Matsuoka, Yusuke" sort="Matsuoka, Yusuke" uniqKey="Matsuoka Y" first="Yusuke" last="Matsuoka">Yusuke Matsuoka</name></author><author><name sortKey="Ueshiba, Hidehiro" sort="Ueshiba, Hidehiro" uniqKey="Ueshiba H" first="Hidehiro" last="Ueshiba">Hidehiro Ueshiba</name></author><author><name sortKey="Komatsu, Nobukazu" sort="Komatsu, Nobukazu" uniqKey="Komatsu N" first="Nobukazu" last="Komatsu">Nobukazu Komatsu</name></author><author><name sortKey="Itoh, Kyogo" sort="Itoh, Kyogo" uniqKey="Itoh K" first="Kyogo" last="Itoh">Kyogo Itoh</name></author><author><name sortKey="Shichijo, Shigeki" sort="Shichijo, Shigeki" uniqKey="Shichijo S" first="Shigeki" last="Shichijo">Shigeki Shichijo</name></author><author><name sortKey="Kanai, Takao" sort="Kanai, Takao" uniqKey="Kanai T" first="Takao" last="Kanai">Takao Kanai</name></author><author><name sortKey="Fukushi, Masaya" sort="Fukushi, Masaya" uniqKey="Fukushi M" first="Masaya" last="Fukushi">Masaya Fukushi</name></author><author><name sortKey="Ishida, Isao" sort="Ishida, Isao" uniqKey="Ishida I" first="Isao" last="Ishida">Isao Ishida</name></author><author><name sortKey="Kirikae, Teruo" sort="Kirikae, Teruo" uniqKey="Kirikae T" first="Teruo" last="Kirikae">Teruo Kirikae</name></author><author><name sortKey="Sasazuki, Takehiko" sort="Sasazuki, Takehiko" uniqKey="Sasazuki T" first="Takehiko" last="Sasazuki">Takehiko Sasazuki</name></author><author><name sortKey="Miyoshi Akiyama, Tohru" sort="Miyoshi Akiyama, Tohru" uniqKey="Miyoshi Akiyama T" first="Tohru" last="Miyoshi-Akiyama">Tohru Miyoshi-Akiyama</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18703211</idno><idno type="pmid">18703211</idno><idno type="doi">10.1016/j.virol.2008.07.012</idno><idno type="wicri:Area/PubMed/Corpus">001A85</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001A85</idno><idno type="wicri:Area/PubMed/Curation">001A85</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001A85</idno><idno type="wicri:Area/PubMed/Checkpoint">001B45</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001B45</idno><idno type="wicri:Area/Ncbi/Merge">001D17</idno><idno type="wicri:Area/Ncbi/Curation">001D17</idno><idno type="wicri:Area/Ncbi/Checkpoint">001D17</idno><idno type="wicri:Area/Main/Merge">003212</idno><idno type="wicri:Area/Main/Curation">003122</idno><idno type="wicri:Area/Main/Exploration">003122</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus.</title><author><name sortKey="Hatakeyama, Seisuke" sort="Hatakeyama, Seisuke" uniqKey="Hatakeyama S" first="Seisuke" last="Hatakeyama">Seisuke Hatakeyama</name><affiliation wicri:level="3"><nlm:affiliation>Department of Infectious Diseases, Research Institute, International Medical Center of Japan, Tokyo, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Infectious Diseases, Research Institute, International Medical Center of Japan, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Matsuoka, Yusuke" sort="Matsuoka, Yusuke" uniqKey="Matsuoka Y" first="Yusuke" last="Matsuoka">Yusuke Matsuoka</name></author><author><name sortKey="Ueshiba, Hidehiro" sort="Ueshiba, Hidehiro" uniqKey="Ueshiba H" first="Hidehiro" last="Ueshiba">Hidehiro Ueshiba</name></author><author><name sortKey="Komatsu, Nobukazu" sort="Komatsu, Nobukazu" uniqKey="Komatsu N" first="Nobukazu" last="Komatsu">Nobukazu Komatsu</name></author><author><name sortKey="Itoh, Kyogo" sort="Itoh, Kyogo" uniqKey="Itoh K" first="Kyogo" last="Itoh">Kyogo Itoh</name></author><author><name sortKey="Shichijo, Shigeki" sort="Shichijo, Shigeki" uniqKey="Shichijo S" first="Shigeki" last="Shichijo">Shigeki Shichijo</name></author><author><name sortKey="Kanai, Takao" sort="Kanai, Takao" uniqKey="Kanai T" first="Takao" last="Kanai">Takao Kanai</name></author><author><name sortKey="Fukushi, Masaya" sort="Fukushi, Masaya" uniqKey="Fukushi M" first="Masaya" last="Fukushi">Masaya Fukushi</name></author><author><name sortKey="Ishida, Isao" sort="Ishida, Isao" uniqKey="Ishida I" first="Isao" last="Ishida">Isao Ishida</name></author><author><name sortKey="Kirikae, Teruo" sort="Kirikae, Teruo" uniqKey="Kirikae T" first="Teruo" last="Kirikae">Teruo Kirikae</name></author><author><name sortKey="Sasazuki, Takehiko" sort="Sasazuki, Takehiko" uniqKey="Sasazuki T" first="Takehiko" last="Sasazuki">Takehiko Sasazuki</name></author><author><name sortKey="Miyoshi Akiyama, Tohru" sort="Miyoshi Akiyama, Tohru" uniqKey="Miyoshi Akiyama T" first="Tohru" last="Miyoshi-Akiyama">Tohru Miyoshi-Akiyama</name></author></analytic><series><title level="j">Virology</title><idno type="eISSN">1096-0341</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Line</term><term>Nucleocapsid (genetics)</term><term>Nucleocapsid (metabolism)</term><term>Nucleocapsid Proteins (chemistry)</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (metabolism)</term><term>RNA, Viral (chemistry)</term><term>RNA, Viral (genetics)</term><term>SARS Virus (chemistry)</term><term>Viral Matrix Proteins (genetics)</term><term>Viral Matrix Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral ()</term><term>ARN viral (génétique)</term><term>Lignée cellulaire</term><term>Nucléocapside (génétique)</term><term>Nucléocapside (métabolisme)</term><term>Protéines de la matrice virale (génétique)</term><term>Protéines de la matrice virale (métabolisme)</term><term>Protéines nucléocapside ()</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (métabolisme)</term><term>Virus du SRAS ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Nucleocapsid Proteins</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Nucleocapsid</term><term>Nucleocapsid Proteins</term><term>RNA, Viral</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Nucléocapside</term><term>Protéines de la matrice virale</term><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid</term><term>Nucleocapsid Proteins</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Nucléocapside</term><term>Protéines de la matrice virale</term><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN viral</term><term>Lignée cellulaire</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">When expressed in mammalian cells, the nucleocapsid (N) and membrane (M) proteins of the severe acute respiratory syndrome coronavirus (SARS-CoV) are sufficient to form pseudoparticles. To identify region(s) of the N molecule required for pseudoparticle formation, we performed biochemical analysis of the interaction of N mutants and M in HEK293 cells. Using a peptide library derived from N, we found that amino acids 101-115 constituted a novel binding site for M. We examined the ability of N mutants to interact with M and form pseudoparticles, and our observations indicated that M bound to NDelta(101-115), N1-150, N151-300, and N301-422, but not to N1-150Delta(101-115). However, pseudoparticles were formed when NDelta(101-115) or N301-422, but not N1-150 or N151-300, were expressed with M in HEK293 cells. These results indicated that the minimum portion of N required for the interaction with M and pseudoparticle formation consists of amino acids 301-422.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Fukushi, Masaya" sort="Fukushi, Masaya" uniqKey="Fukushi M" first="Masaya" last="Fukushi">Masaya Fukushi</name><name sortKey="Ishida, Isao" sort="Ishida, Isao" uniqKey="Ishida I" first="Isao" last="Ishida">Isao Ishida</name><name sortKey="Itoh, Kyogo" sort="Itoh, Kyogo" uniqKey="Itoh K" first="Kyogo" last="Itoh">Kyogo Itoh</name><name sortKey="Kanai, Takao" sort="Kanai, Takao" uniqKey="Kanai T" first="Takao" last="Kanai">Takao Kanai</name><name sortKey="Kirikae, Teruo" sort="Kirikae, Teruo" uniqKey="Kirikae T" first="Teruo" last="Kirikae">Teruo Kirikae</name><name sortKey="Komatsu, Nobukazu" sort="Komatsu, Nobukazu" uniqKey="Komatsu N" first="Nobukazu" last="Komatsu">Nobukazu Komatsu</name><name sortKey="Matsuoka, Yusuke" sort="Matsuoka, Yusuke" uniqKey="Matsuoka Y" first="Yusuke" last="Matsuoka">Yusuke Matsuoka</name><name sortKey="Miyoshi Akiyama, Tohru" sort="Miyoshi Akiyama, Tohru" uniqKey="Miyoshi Akiyama T" first="Tohru" last="Miyoshi-Akiyama">Tohru Miyoshi-Akiyama</name><name sortKey="Sasazuki, Takehiko" sort="Sasazuki, Takehiko" uniqKey="Sasazuki T" first="Takehiko" last="Sasazuki">Takehiko Sasazuki</name><name sortKey="Shichijo, Shigeki" sort="Shichijo, Shigeki" uniqKey="Shichijo S" first="Shigeki" last="Shichijo">Shigeki Shichijo</name><name sortKey="Ueshiba, Hidehiro" sort="Ueshiba, Hidehiro" uniqKey="Ueshiba H" first="Hidehiro" last="Ueshiba">Hidehiro Ueshiba</name></noCountry><country name="Japon"><region name="Région de Kantō"><name sortKey="Hatakeyama, Seisuke" sort="Hatakeyama, Seisuke" uniqKey="Hatakeyama S" first="Seisuke" last="Hatakeyama">Seisuke Hatakeyama</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003122 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003122 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:18703211
|texte= Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:18703211" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |